Compositions containing hc-ha/ptx3 complexes and methods of use thereof
AssigneeTissuetech, Inc.
InventorsScheffer TSENG, Hua HE, Sean TIGHE, Suzhen ZHANG, Ying-Tieng ZHU
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Abstract
Provided herein are methods for the production of native and reconstituted hyaluronan (HA) complexes containing pentraxin-3 (PTX3) and heavy chain 1 (HC1) of inter alpha inhibitor (IαI). Compositions containing the complexes and therapeutic methods using the complexes are provided. Combinations and kits for use in practicing the methods also are provided.
Claims148
A method for producing a reconstituted HC-HA/PTX3 (rcHC-HA/PTX3) complex in vitro, comprising contacting (i) high molecular weight hyaluronan (HMW HA) immobilized to a solid support, (ii) pentraxin 3 (PTX3) protein, (iii) inter-α-inhibitor (IαI) protein comprising heavy chain 1 (HC1) and (iv) Tumor necrosis factor α-stimulated gene 6 (TSG-6) to form an immobilized rcHC-HA/PTX3 complex.
A reconstituted HC-HA (rcHC-HA/PTX3) complex comprising high molecular weight hyaluronan (HMW HA), (ii) pentraxin 3 (PTX3) protein, and (iii) inter-α-inhibitor (IαI) protein comprising heavy chain 1 (HC1).
An isolated native HC-HA/PTX3 (nHC-HA/PTX3) complex purified from an amniotic membrane extract or an umbilical cord extract.
A method for inducing the M2 polarization of macrophages comprising contacting an LPS-stimulated macrophage with an rcHC-HA/PTX3 or isolated nHC-HA/PTX3 complex.
A method for inducing or maintaining pluripotency in a cell, comprising culturing the cell with an nHC-HA/PTX3 complex or an rcHC-HA/PTX3 complex, thereby inducing or maintaining pluripotency in the cell.
Dependencies are inferred from the claim text.