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US 2019/0169229 A1Published application
Tangential flow filtration based protein refolding methods
AssigneeBristol-Myers Squibb Company
InventorsBenjamin C. BLUM, Cristopher HOLLANDER
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Abstract
Provided herein are methods for refolding proteins that are denatured. Exemplary methods comprise solubilizing the denatured protein with a denaturing agent, e.g., a chaotropic agent, and renaturing the protein using a buffer exchanging system, e.g., tangential flow filtration (TFF).
Claims28
A method for refolding a denatured protein, comprising
- combining denatured protein with a solubilization buffer to obtain a first protein composition comprising solubilized denatured protein;
- diafiltering the first protein composition comprising solubilized denatured protein with 2-4 diavolumes of a refold buffer, to obtain a second protein composition comprising partially refolded protein;
- and
- incubating the second protein composition comprising partially refolded protein with a refold/oxidizing buffer to obtain a third protein composition comprising the protein in a refolded state.
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Dependencies are inferred from the claim text.