Cell co-culture chip and process for the production thereof

A microfluidic cell culture chip which contains a central module comprising:

• a central unit, which contains
• a support consisting of a non-resorbable membrane, comprising an upper face and a lower face, perforated by at least one perforation, said support being composed, in particular of bioprinted plastic, polycarbonate, tissue culture plastic, glass or SU8,
• a 3D nanostructured porous membrane, comprising an upper face and a lower face, and comprising at least one protuberance, said at least one protuberance comprising an outer face and an inner face and forming a relief structure on the side of the upper face of the 3D nanostructured membrane, said protuberance being, in particular in the shape of a hollow dome having a circular base,
• said lower face of said 3D nanostructured porous membrane being positioned secured to said upper face of said support,
• or said upper face of the 3D nanostructured membrane being positioned secured to said lower face of said support,
• and said 3D nanostructured porous membrane and the at least one protuberance being composed of materials, suitable for the culture of two distinct cell types;
• a base,
• said central unit being integrated in said base, and forming a whole with said base.