System and method for determining efficacy and dosage using parallel/serial dual microfluidic chip

A method for testing a treatment agent for a predetermined biologic material, comprising:

• receiving a biologic sample, the biologic sample containing a unique combination of the predetermined biologic material that must be treated via one of a plurality of treatment agents and a patient's biologic material;
• testing a first portion of the biologic sample containing the unique combination of the predetermined biologic material and the patient's biologic material in a plurality of first parallel testing pathways, each of the plurality of first parallel testing pathways applying a different treatment agent to the first portion of the biologic sample;
• determining a treatment agent providing a best treatment efficacy responsive to the tests performed in each of the plurality of testing pathways;
• testing a second portion of the biologic sample containing the unique combination of the predetermined biologic material and the patient's biologic material in a selected pathway of a second plurality of testing pathways, the selected pathway selected responsive to the determined treatment agent providing the best treatment efficacy, the selected pathway applying at least one dosage level of the determined treatment agent providing the best treatment efficacy to the second portion of the biologic sample;
• determine a lowest dosage level of the determined treatment agent providing the best treatment efficacy responsive to the applied at least one dosage level;
• and
• providing an indication of the determined treatment agent and the determined lowest dosage level.

A method for determining a treatment agent and dosage level for a predetermined biologic material, comprising:

• receiving a biologic sample, the biologic sample containing the predetermined biologic material for treatment by one of a plurality of treatment agents;
• holding the biologic sample containing the predetermined biologic material within a first reservoir;
• pumping a portion of the biologic sample into each of a first plurality of parallel pathways from the first reservoir using a micro-pump;
• applying a separate treatment agent of the plurality of treatment agents within each of the first plurality of parallel pathways to the portion of the biologic sample within the parallel pathway;
• holding the portion of the biologic sample treated with one of a plurality of treatment agents in a plurality of second reservoirs;
• detecting efficacy of the plurality of treatment agents on the biologic material within the biologic sample through a plurality of second viewing windows each associated with one of the plurality of second reservoirs.
• determining the treatment agent of the plurality of treatment agents providing a best treatment efficacy for the predetermined biologic material within the biologic sample responsive to the detected efficacy of the plurality of treatment agents applied to the portion of the biologic sample;
• pumping a second portion of the biologic sample into a selected second parallel pathway associated with the determined treatment agent of a second plurality of parallel pathways from the first reservoir using a second micro-pump;
• applying the determined treatment agent at a plurality of different dosage levels within the selected second parallel pathway to the second portion of the biologic sample within the second parallel pathway;
• holding the second portion of the biologic sample treated with the treatment agent determined to provide the best treatment efficacy with the at least one dosage level in a plurality of third reservoirs;
• detecting efficacy of the at least one dosage level of the determined treatment agent on the biologic material within the biologic sample through at least one second viewing windows each associated with one of the plurality of third reservoirs;
• determining a dosage level of the plurality of different dosage levels of the determined treatment agent with respect to the predetermined biologic material providing the best treatment efficacy;
• and
• providing an output indicating the treatment agent and the dosage level of the treatment agent providing the best treatment efficacy.