Optogenetic control of endothelial cells
AssigneesBrown University, Massachusetts Institute of Technology
InventorsTyler C. BROWN, Christopher I. MOORE
Life of this patent
Estimating the term…
Abstract
The invention features methods for regulating vascular properties by controlling the membrane properties of endothelial cells using optogenetics and light. The invention features methods to transport therapeutics across the vascular barrier into tissues such as the brain and the lung, with high spatial and temporal precision, and for controlling vascular properties such as vascular tone, arterial diameter, and vascular growth.
Claims24
A method for changing the permeability of endothelial cells, said method comprising the step of contacting endothelial cells expressing optogenetic reagents with light, wherein said light activates said optogenetic reagents and thereby changes the permeability of said endothelial cells.
A method for changing the permeability of endothelial cells, said method comprising:
- a) infecting endothelial cells with recombinant viruses comprising a recombinant nucleic acid encoding said optogenetic reagent to produce infected endothelial cells;
- and b) contacting said infected endothelial cells with light, wherein said light activates said optogenetic reagents and thereby changes permeability of said endothelial cells.
(canceled)
5-6. (canceled)
(canceled)
13-15. (canceled)
21-22. (canceled)
24-29. (canceled)
(canceled)
Dependencies are inferred from the claim text.