Systems and methods of radioprotection of allografts
AssigneeThe General Hospital Corporation
InventorsEbru ORAL, Jeremy SUHARDI, Orhun MURATOGLU
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Abstract
Systems and methods of protecting allograft against radiation damage are disclosed. Systems and methods of incorporating additives such as radioprotectants into allograft tissue are also disclosed. The systems and methods comprise providing an allograft; cleaning the allograft; contacting the allograft with at least one radioprotectant, thereby obtaining a radioprotectant-doped allograft; contacting the radioprotectant-doped allograft with a supercritical fluid, thereby obtaining a radioprotectant doped and homogenized allograft.
Claims74
A method of incorporating a radioprotectant into an allograft, the method comprising:
- a. providing an allograft;
- b. cleaning the allograft;
- c. contacting the allograft with at least one radioprotectant, thereby obtaining a radioprotectant-doped allograft;
- and
- d. contacting the radioprotectant-doped allograft with a supercritical fluid thereby obtaining a radioprotectant doped and homogenized allograft.
A method of making a radiation sterilized allograft, the method comprising:
- a. providing an allograft;
- b. cleaning the allograft;
- c. contacting the allograft with at least one radioprotectant(s), thereby obtaining a radioprotectant-doped allograft;
- d. contacting the radioprotectant-doped allograft with a supercritical fluid;
- thereby obtaining a radioprotectant doped and homogenized allograft;
- and
- e. irradiating the radioprotectant doped and homogenized allograft, thereby obtaining a radiation sterilized allograft.
A method of making a medical implant, the method comprising:
- a. providing an allograft;
- b. cleaning the allograft;
- c. contacting the allograft with radioprotectant(s), thereby obtaining a radioprotectant-doped allograft;
- d. contacting the radioprotectant-doped allograft with a supercritical fluid thereby obtaining a radioprotectant doped and homogenized allograft;
- and
- e. irradiating the radioprotectant doped and homogenized allograft.
A method of incorporating radioprotectant into allografts, the method comprising:
- a. providing a allograft;
- b. cleaning allograft;
- c. contacting allograft with radioprotectant(s) and at least one more additive, thereby obtaining a radioprotectant-doped allograft;
- and
- d. contacting the radioprotectant-doped allograft with a supercritical fluid thereby obtaining a radioprotectant doped and homogenized allograft.
A method of making a radiation sterilized allograft, the method comprising:
- a. providing an allograft;
- b. cleaning allograft;
- c. contacting allograft with radioprotectant(s) and at least one more additive, thereby obtaining a radioprotectant-doped allograft;
- d. contacting the radioprotectant-doped allograft with a supercritical fluid thereby obtaining a radioprotectant doped and homogenized allograft;
- and
- e. irradiating the radioprotectant doped and homogenized allograft.
A method of making a medical implant, the method comprising:
- a. providing an allograft;
- b. cleaning allograft;
- c. contacting allograft with radioprotectant(s) and at least one more additive, thereby obtaining a radioprotectant-doped allograft;
- d. contacting the radioprotectant-doped allograft with a supercritical fluid thereby obtaining a radioprotectant doped and homogenized allograft;
- and
- e. irradiating radioprotectant doped and homogenized allograft.
The method according to claim 25, wherein the bone allograft is cancellous or cortical.
A method of protecting collagen cross-links in a radiation sterilized allograft, the method comprising:
- a. providing an allograft;
- b. cleaning the allograft;
- c. contacting the allograft with at least one radioprotectant thereby creating a radioprotectant-doped allograft;
- d. contacting the radioprotectant-doped allograft with a supercritical fluid thereby obtaining a radioprotectant doped and homogenized allograft;
- and
- e. irradiating the radioprotectant doped and homogenized allograft.
A method of making a medical implant with preserved collagen cross-linking, the method comprising:
- a. providing an allograft;
- b. cleaning the allograft;
- c. contacting the allograft with radioprotectant(s);
- d. contacting the radioprotectant-doped allograft with a supercritical fluid thereby obtaining a radioprotectant doped and homogenized allograft;
- and
- e. irradiating the radioprotectant doped and homogenized allograft.
A prosthetic system for implantation in a subject, the prosthetic system comprising:
- radioprotectant-doped allograft(s).
A method of making an allograft with improved integration to host tissue, the method comprising:
- a. providing an allograft;
- b. cleaning the allograft;
- c. contacting the allograft with at least one radioprotectant, thereby obtaining a radioprotectant-doped allograft;
- and
- d. contacting the radioprotectant-doped allograft with a supercritical fluid thereby obtaining a radioprotectant doped and homogenized allograft;
- and
- e. Irradiating the radioprotectant doped and homogenized allograft.
An allograft with improved integration to host tissue made by a method comprising:
- a. providing an allograft;
- b. cleaning the allograft;
- c. contacting the allograft with at least one radioprotectant and optionally other additive(s), thereby obtaining a radioprotectant-doped allograft;
- and
- d. contacting the radioprotectant-doped allograft with a supercritical fluid optionally in the presence of other additive(s);
- thereby obtaining a radioprotectant doped and homogenized allograft;
- and
- e. irradiating the radioprotectant doped and homogenized allograft.
A method of making an allograft with improved integration to host tissue, the method comprising:
- a. providing an allograft;
- b. cleaning the allograft;
- c. contacting the allograft with at least one radioprotectant and optionally other additive(s), thereby obtaining a radioprotectant-doped allograft;
- and
- d. contacting the radioprotectant-doped allograft with a supercritical fluid optionally in the presence of other additive(s);
- thereby obtaining a radioprotectant doped and homogenized allograft;
- and
- e. irradiating the radioprotectant doped and homogenized allograft.
A method of making an allograft with improved integration to host tissue, the method comprising:
- a. providing an allograft;
- b. cleaning the allograft;
- c. contacting the allograft with at least one radioprotectant and at least one antibiotic, thereby obtaining a radioprotectant and antibiotic-doped allograft;
- and
- d. contacting the radioprotectant-doped allograft with a supercritical fluid;
- thereby obtaining a radioprotectant and antibiotic-doped and homogenized allograft;
- and
- e. irradiating the radioprotectant and antibiotic-doped and homogenized allograft.
A method according to any of the claims, wherein the doping is done at room temperature.
A method according to any of the claims, wherein the doping is done between room temperature and 60° C.
A method according to any of the claims, wherein the homogenization is done at about 40° C.
A method according to any of the claims, wherein the doping is done under inert atmosphere.
A method according to any of the claims wherein the homogenization in supercritical carbon dioxide is done at about 85 bar.
A method according to any of the claims, wherein the doping is done for about 6 hours.
A method according to any of the claims, wherein the doping is done for less than 6 hours.
A method according to any of the claims, wherein the homogenization in supercritical fluid is done for about 24 hours.
A method according to any of the claims, wherein the homogenization in supercritical carbon dioxide is done for less than 24 hours.
Dependencies are inferred from the claim text.