Mesenchymal stem cell-mediated functional tooth regeneration

A stem cell composition, comprising:

• a plurality of human apical papilla stem cells (SCAP);
• wherein the SCAP are:
• isolated from root apical papilla of a tooth extracted from an adult human;
• characterized by expression of STRO-1, CD146, CD166 and CD24-positive surface markers;
• and
• capable of differentiating into odontoblastic or osteoblastic cells.

A stem cell composition, comprising:

• two stem cell compositions, wherein the first stem cell composition comprises plurality of human apical papilla stem cells (SCAP), and the second stem cell composition comprises human periodontal ligament stem cells (PDLSC);
• wherein the SCAP are:
• isolated from root apical papilla of a tooth extracted from an adult human;
• characterized by expression of STRO-1, CD146, CD166 and CD24-positive surface markers;
• and
• capable of differentiating into odontoblastic or osteoblastic cells.