Aligned fiber and method of use thereof

A method of treating a tissue defect comprising applying a scaffold comprising one or more electrospun fibers, wherein a fast Fourier transform (FFT) analysis result of the fibers have adjacent major peaks with about 180° apart from each other, and wherein the scaffold is in a form of one or more elongated sheets, to surround the tissue defect.

A method of treating a tissue defect or gap comprising applying a scaffold comprising one or more electrospun fibers, wherein a fastlourier transform (FFT) analysis result of the fibers have adjacent major peaks with about 180° apart from each other, and wherein the scaffold is in the form of one or more elongated rolls, between the tissue defect or gap.

A method of cell culture comprising culturing cells on a scaffold comprising one or more electrospun fibers, wherein a fast Fourier transform (FFT) analysis result of the fibers have adjacent major peaks with about 180° apart from each other in conditions suitable for the cell culture.

A method of promoting differentiation of stem cells into osteoblasts, chondrocytes, ligament or tendon, the method comprising culturing the cells on a scaffold comprising one or more electrospun fibers, wherein a fast Fourier transform (FFT) analysis result of the fibers have adjacent major peaks with about 180° apart from each other in conditions suitable for the cell differentiation,