Serum fraction of platelet-rich fibrin as a cell culture additive

A method for using an isolated serum fraction of platelet rich fibrin (SPRF) as a cell culture additive, said method comprising the steps of

• culturing cells by incubating said cells in a medium, and
• adding the SPRF to the medium,
• said SPRF being obtained by a method comprising the steps of
• a. separating and removing the red blood cell fraction from a venous blood sample without the addition of an anticoagulant, to provide a plasma;
• b. clotting said plasma to obtain a coagel of PRF spontaneously by centrifugation carried out at 1000 to 5000 g and a supernatant, wherein in said method the centrifugation is carried out for 2 to 20 minutes;
• c. pressing or squeezing the coagel to obtain fluid fraction from the coagel, thereby obtaining said SPRF;
• said SPRF comprising a platelet releasate from activated platelets and
• said SPRF comprising a reduced content of red blood cells, platelets or fibrinogen as compared to whole blood or a reduced content of fibrin as compared to said plasma, and
• wherein said SPRF is capable of inducing cell proliferation or restoring cell proliferation capacities.

A medium comprising

• a cell culture medium and
• a serum fraction containing the fluid fraction of platelet-rich fibrin, i.e. serum fraction of PRF (SPRF),
• wherein
• said SPRF being obtained by a method comprising the steps of
• a. separating and removing the red blood cell fraction from a venous blood sample to provide a plasma without the addition of an anticoagulant;
• b. clotting said plasma to obtain a coagel of PRF spontaneously by centrifugation carried out at 1000 to 5000 g and a supernatant, wherein in said method the centrifugation is carried out for 2 to 20 minutes;
• c. pressing or squeezing the coagel to obtain fluid fraction from the coagel, thereby obtaining said SPRF;
• wherein said SPRF is added to the cell culture medium,
• said SPRF comprising a platelet releasate from activated platelets and
• said SPRF comprising a reduced content of red blood cells, platelets or fibrinogen as compared to whole blood or a reduced content of fibrin as compared to said plasma, and
• wherein said SPRF is capable of inducing cell proliferation or restoring cell proliferation capacities.