Cryopreservation of cartilage and osteochondral tissue

Abstract

Provided are systems and methods for cryopreserving tissue, particularly cartilage tissue and osteochondral tissue. Also provided are tissue products made using such systems and methods. Certain methods involve combining tissue with a cryopreservation solution in a processing vessel and applying resonant acoustic energy thereto prior to freezing the tissue. The resonant acoustic energy rapidly agitates the tissue with the cryopreservation solution by vibration. By applying resonant acoustic energy to the tissue during processing, the rate or efficiency of processing, or both, may be improved. Certain methods involve soaking tissue in a cryopreservation solution prior to freezing the tissue.

Dependencies are inferred from the claim text.