Biological substance quantitation method, pathological diagnosis support system and recording medium storing computer readable program

A pathological diagnosis support system which quantitates an amount of a target substance in a sample stained with a staining reagent which stains the target substance with a fluorescent substance, the system comprising:

• a storage which stores a program for quantitating the amount of the target substance;
• and
• a processor, the program causing the processor to function as:
• a fluorescent image inputting unit which inputs a fluorescent image which represents a presence of the target substance in the sample by a fluorescent bright spot;
• a fluorescence quantitation unit which calculates an evaluation value by quantitative evaluation of the fluorescent bright spot in the fluorescent image;
• a standard fluorescent image inputting unit which inputs a standard fluorescent image under a same condition as the fluorescent image inputting unit, wherein
• the standard fluorescent image represents a presence of the target substance in a standard sample by a fluorescent bright spot based on staining under a same condition as the sample, and
• an amount of the target substance in the standard sample is measured in advance;
• a standard fluorescent quantitation unit which calculates an evaluation value by quantitative evaluation of the fluorescent bright spot in the standard fluorescent image under a same condition as the fluorescence quantitation unit;
• a correlation calculator which calculates a correlation between the amount of the target substance in the standard sample and the evaluation value of the fluorescent bright spot in the standard fluorescent image;
• and
• a converter which converts the evaluation value of the fluorescent bright spot in the fluorescent image to an amount of the target substance in the sample based on the correlation.

A non-transitory recording medium storing a computer readable program causing a computer which quantitates an amount of a target substance in a sample stained with a staining reagent which stains the target substance with fluorescent substances, to function as:

• a fluorescent image inputting unit which inputs a fluorescent image which represents a presence of the target substance in the sample by a fluorescent bright spot;
• a fluorescence quantitation unit which calculates an evaluation value by quantitative evaluation of the fluorescent bright spot in the fluorescent image;
• a standard fluorescent image inputting unit which inputs a standard fluorescent image under a same condition as in the fluorescent image inputting unit, wherein
• the standard fluorescent image represents a presence of the target substance in a standard sample by a fluorescent bright spot based on staining under a same condition as the sample, and
• an amount of the target substance in the standard sample is measured in advance;
• a standard fluorescent quantitation unit which calculates an evaluation value by quantitative evaluation of the fluorescent bright spot in the standard fluorescent image under a same condition as the fluorescence quantitation unit;
• a correlation calculator which calculates a correlation between the amount of the target substance in the standard sample and the evaluation value of the fluorescent bright spot in the standard fluorescent image;
• and
• a converter which converts the evaluation value of the fluorescent bright spot in the fluorescent image to an amount of the target substance in the sample based on the correlation.