Assays

A method for detecting a human immunodeficiency virus (HIV) target nucleic acid in a liquid, comprising:

• a) introducing a liquid into a reaction chamber of a microfluidic device, the liquid comprising an HIV polynucleotide, binding the HIV polynucleotide to a first binding member located within the reaction chamber;
• b) amplifying the HIV polynucleotide within the reaction chamber to form amplicons, the amplification being performed in the presence of a reporter compound, binding a subset of the reporter compound to amplicons within the reaction chamber, binding a remaining subset of the reporter compound to a second binding member within the reaction chamber, and detecting the remaining subset of the reporter compound bound to the second binding member;
• and
• c) determining a value indicative of the presence and/or amount of reporter compound captured on the second binding member;
• and determining a value indicative of the amount of target nucleic acid based on the value indicative of the amount of reporter compound captured on the second binding member.