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AssigneeAlliance for Sustainable Energy, LLC
InventorsGregg Tyler BECKHAM, Christopher W. JOHNSON, Derek R. VARDON
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An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.
A non-naturally occurring Pseudomonas not capable of expressing 2-pyrone-4,6-dicarboxylic acid hydrolase, and capable of producing 2-oxo-2H-pyran-4,6-dicarboxylic acid, wherein the Pseudomonas is capable of growing on at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and wherein the Pseudomonas lacks the gene encoding for LigI.
Dependencies are inferred from the claim text.