Enzymatic Hydroxylation of Aliphatic Hydrocarbon

18:

• A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted, linear or branched, aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3, comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide having peroxygenase activity;
• wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:
• 1.

19:

• The method of claim 18, wherein the carbon in position 2 or 3, which is hydroxylated, is unsubstituted until it is contacted with the peroxygenase.

20:

• The method of claim 18, wherein the amino acid sequence of the polypeptide comprises the motif of SEQ ID NO:
• 9.

21:

• The method of claim 18, wherein the amino acid sequence of the polypeptide comprises the motif of SEQ ID NO:
• 10.

22:

• The method of claim 18, wherein the amino acid sequence of the polypeptide comprises the motif of SEQ ID NO:
• 11.

23:

• The method of claim 18, wherein the amino acid sequence of the polypeptide comprises the motif of SEQ ID NO:
• 12.

24:

• The method of claim 18, wherein the amino acid sequence of the polypeptide comprises the motif of SEQ ID NO:
• 13.

25:

• The method of claim 18, wherein the amino acid sequence of the polypeptide comprises the motif of SEQ ID NO:
• 14.

26:

• The method of claim 18, wherein the polypeptide has at least 95% sequence identity to SEQ ID NO:
• 1.

27:

• The method of claim 18, wherein the polypeptide comprises or consists of the amino acid sequence of SEQ ID NO:
• 1.

28:

• The method of claim 18, wherein the polypeptide is a fragment of SEQ ID NO:
• 1 having peroxygenase activity.

29:

• The method of claim 18, wherein the substituents of the aliphatic hydrocarbon are selected from the group consisting of halogen, hydroxyl, carboxyl, amino, nitro, cyano, thiol, sulphonyl, formyl, acetyl, methoxy, ethoxy, phenyl, benzyl, xylyl, carbamoyl and sulfamoyl.

30:

• The method of claim 18, wherein the substituents are selected from the group consisting of chloro, hydroxyl, carboxyl and sulphonyl;
• in particular chloro and carboxyl.

31:

• The method of claim 18, wherein the aliphatic hydrocarbon is unsubstituted.

32:

• The method of claim 18, wherein the aliphatic hydrocarbon is linear.

33:

• The method of claim 18, wherein the aliphatic hydrocarbon is an alkane.

34:

• The method of claim 33, wherein the alkane is propane, butane, pentane, hexane, heptane, octane, nonane or decane, or isomers thereof.

35:

• The method of claim 18, wherein the aliphatic hydrocarbon is part of a fatty acid.

36:

• A method for hydroxylation in position 2 or 3 of the terminal end of an acyl group of a lipid, comprising contacting the lipid with hydrogen peroxide and a polypeptide having peroxygenase activity;
• wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:
• 1.

37:

• The method of claim 36, wherein the polypeptide has at least 95% identity to SEQ ID NO:
• 1.