Plasmid Constructs For Heterologous Protein Expression And Methods Of Use
AssigneeOncoSec Medical Incorporated
InventorsJean Campbell, David A. Canton, Robert H. Pierce
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Abstract
Provided are plasmid vector constructs encoding multiple immunomodulatory proteins where each protein or component thereof can be expressed utilizing appropriate promotors and/or translation modifiers. Additional immunomodulatory proteins and genetic adjuvants containing shared tumor antigens can be added to further therapeutic potential as well as allow tracking of therapeutic treatment. Also provides are methods of expressing the plasmid constructs.
Claims21
An expression plasmid construct comprising a plurality of expression cassettes defined by the formula:
- P-A-T-B
- wherein:
- a) P is an expression promoter;
- b) A and B encode immunomodulatory molecules;
- and
- c) T is a translation modification element.
An expression plasmid comprising a plurality of expression cassettes defined by the formula:
- P-A-T-A′-T-B or P-B-T-A-T-A′
- wherein:
- a) P is an expression promoter;
- b) A and A′ are chains of a heterodimeric cytokine;
- c) B is at least one genetic adjuvant fused to at least one antigen;
- and
- d) T is a translation modification element.
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11-15. (canceled)
An expression plasmid construct comprising a plurality of expression cassettes defined by the formula:
- P-A-T-A′
- wherein:
- a) P is an expression promoter;
- b) A, and A′ encode subunits of an immunomodulatory molecule;
- and
- c) T is a translation modification sequence.
An assay to determine improved response to a checkpoint inhibitor comprising:
- a) injecting a plasmid encoding IL-12 into a tumor on a subject;
- b) electroporating the tumor at a voltage sufficient for transfection of the plasmid into at least one cell in the tumor;
- c) administering a checkpoint inhibitor systemically;
- d) analyzing the tumor for immune stimulatory gene expression;
- and
Dependencies are inferred from the claim text.