Culture medium for use in differentiation of pluripotent stem cell into neural stem cell, and use thereof
AssigneeKeio University
InventorsHideyuki OKANO, Wado AKAMATSU, Koki FUJIMORI, Tomoko NODA, Takayuki ANDOH, Toshiki TEZUKA, Takuya MATSUMOTO
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Abstract
A method is provided for uniformly differentiating a pluripotent stem cell into a neural stem cell, with elimination of variation among cell strains or clones of the pluripotent stem cell and without formation of an embryoid body, regardless of the origin of the pluripotent stem cell
Claims18
A method for uniformly differentiating a pluripotent stem cell into a neural stem cell, with elimination of variation among cell strains or clones of the pluripotent stem cell and without formation of an embryoid body, regardless of the origin of the pluripotent stem cell, comprising:
- culturing the pluripotent stem cell in a culture medium containing a fibroblast growth factor 2 (FGF 2), a rho-associated protein kinase (ROCK) inhibitor, and a leukemia inhibitory factor (LIF) as active components.
(canceled)
A method for improving the efficiency of differentiating a pluripotent stem cell into a neural stem cell, the method comprising:
- culturing the pluripotent stem cell in a culture medium containing FGF2, a ROCK inhibitor, and LIF as active components.
Dependencies are inferred from the claim text.