Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
AssigneeVentana Medical Systems, Inc.
InventorsMichael Farrell, Antony Hubbard, Eric May, Lei Tang, Tsu-Shuen Tsao, Wenjun Zhang
Life of this patent
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Abstract
The present disclosure is directed to epitope-tagged antibodies, as well as methods of employing the epitope-tagged antibodies for detecting one or more targets in a biological sample, e.g. a tissue sample.
Claims30
An epitope-tagged antibody comprising an antibody and at least one epitope tag construct, the epitope tag construct comprising alternating spacers and epitope tags.
An epitope tag construct comprising tandem repeat epitope tags separated by spacers, the epitope tag construct comprising from 2 to 6 epitope tags, the epitope tags comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, and SEQ ID NO:
- 9;
- and wherein at least one of the spacers separating the epitope tags comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13, and SEQ ID NO:
- 14.
Dependencies are inferred from the claim text.