Organisms and biosynthetic processes for hydrocarbon synthesis
AssigneeInvista North America S.A.R.L.
InventorsPaul S. PEARLMAN, Alex Van Eck CONRADIE, Gary SMITH
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Abstract
Methods for biosynthesising hydrocarbons from a gaseous substrate in non-naturally occurring acetogens as well as non-naturally occurring acetogens for production of hydrocarbons are provided.
Claims45
A non-naturally occurring acetogen capable of producing hydrocarbons or derivatives thereof from a gaseous substrate, said acetogen comprising:
- (i) an alteration of at least one polynucleotide, the polynucleotide encoding a polypeptide having an activity of an alpha-acetolactate decarboxylase or encoding a polypeptide having an activity of a lactate dehydrogenase, or
- (ii) an alteration of at least two polynucleotides, wherein a first polynucleotide encodes a polypeptide having an activity of an alpha-acetolactate decarboxylase and a second polynucleotide encodes a polypeptide having an activity of a lactate dehydrogenase,
- wherein the polynucleotide alteration eliminates the activity of the encoded polypeptide.
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A composition comprising a means for producing a hydrocarbon via a beta-ketothiolase route via pyruvate via ldh, a 2-hydroxyacyl-CoA dehydratase route via ldh, a 2-hydroxyacyl-CoA dehydratase route via mdd or a polyketide synthase route utilizing ldh.
A genetic construct comprising at least one polynucleotide encoding a polypeptide having an activity of an alpha-acetolactate decarboxylase and/or encoding a polypeptide having an activity of a lactate dehydrogenase, wherein said polynucleotide is altered to eliminate activity of the encoded polypeptide.
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A bio-derived, bio-based, or fermentation-derived product or derivative thereof produced according to any of FIGS. 1-4.
Dependencies are inferred from the claim text.