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US 2019/0056350 A1Published application
Image capillary isoelectric focusing to analyze protein variants in a sample matrix
OwnerAnu RAMBHADRAN, Peng CUI +5inventors
InventorsAnu RAMBHADRAN, Peng CUI, Paul BIGWARFE, Michele LASTRO, Clare RYAN, Junyu MA, Kun LU
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Abstract
Embodiments of the present disclosure are directed to methods, systems, devices and kits corresponding to a method for analyzing charge variants of a protein such as vascular endothelial growth factor VEGF-Trap.
Claims23
A method for analyzing charge variants of vascular endothelial growth factor VEGF-Trap comprising:
- loading a protein sample onto a separation capillary comprising a mixture of at least a carrier ampholyte, methylcellulose, and a stabilizing additive;
- applying a first voltage for a first predetermined period of time such that the carrier ampholyte forms a pH gradient within the capillary;
- applying a second voltage for a second predetermined period of time to focus the migration of charge variants of the protein within the capillary such that the overall charge of the variants is neutral;
- and
- detecting and quantifying charge variants of the protein.
2 more elements
An iCIEF capillary tube configured for use in a charge variant analysis of VEGF-Trap comprising:
- a capillary tube configured to receive a protein, and configured with a mixture of carrier ampholyte, methylcellulose, and a stabilizing additive,
- wherein the capillary tube includes a fluorocarbon coating.
Dependencies are inferred from the claim text.