Method and device for measuring lifespan of red blood cell

A method for measuring a lifespan of a red blood cell employing at least one gas channel system each comprising a gas channel;

• a pulmonary alveolus gas bag, a background gas bag, a first reversing gas bag and a second reversing gas bag respectively connected to the gas channel via a respective solenoid valve;
• wherein each gas channel is connected to a same CO gas chamber, CO2 gas chamber, cylinder piston assembly and air pump, wherein both of the CO gas chamber and the CO2 gas chamber have an exhaust port and an inlet port with a solenoid valve, wherein the method for measuring the lifespan of a red blood cell comprises following steps implemented sequentially:
• S1) controlling the air pump to pump external air into the gas channel through a drying tube and a catalytic tube and finally into the CO2 gas chamber through its inlet port, and then expelling the air in the CO2 gas chamber through the exhaust port of the CO2 gas chamber in a first time duration;
• S2) controlling the cylinder piston assembly to deliver pulmonary alveolus gas from the pulmonary alveolus gas bag into the CO2 gas chamber through the gas channel for determining its CO2 concentration;
• S3) controlling the cylinder piston assembly to deliver the pulmonary alveolus gas from the pulmonary alveolus gas bag into the first reversing gas bag through the gas channel;
• S4) controlling the air pump to pump the external air into the gas channel through the drying tube and the catalytic tube and finally into the CO2 gas chamber, and then discharge the same from the exhaust port of the CO2 gas chamber in the first time duration;
• S5) controlling the cylinder piston assembly to deliver background gas from the background gas bag into the second reversing gas bag through the gas channel;
• S6) controlling the air pump to pump the external air into the gas channel through the drying tube and the catalytic tube and finally into the CO2 gas chamber through its inlet port, and then expelling the air in the CO2 gas chamber through the exhaust port of the CO2 gas chamber in the first time duration;
• wherein the method for measuring the lifespan of a red blood cell further comprising following steps S7-S11 implemented sequentially on each gas channel system after the implementation of the steps S1-S6:
• S7) controlling the air pump to pump the external air into the gas channel through the drying tube and the catalytic tube and finally into the CO gas chamber through its inlet port, and then expelling the air in the CO gas chamber through the exhaust port of the CO gas chamber in a second time duration;
• S8) delivering the background gas in the second reversing gas bag to the CO gas chamber with a small amount each time at an uniform velocity, or for multiple times of intermittence with a small amount each time at an uniform velocity for determining its CO concentration;
• wherein each time of gas delivery has a third time duration, and each intermittence of the multiple injections has a fourth time duration;
• S9) controlling the air pump to pump the external air into the gas channel through the drying tube and the catalytic tube and finally into the CO gas chamber through its inlet port, and then expelling the air in the CO gas chamber through the exhaust port of the same CO gas chamber in a second time duration;
• S10) controlling the cylinder piston assembly to deliver the pulmonary alveolus gas in the first reversing gas bag to the CO gas chamber with a small amount each time at an uniform velocity, or for multiple times of intermittence with a small amount each time at an uniform velocity for determining its CO concentration;
• wherein each time of gas delivery has the third time duration, and each intermittence of the multiple injections has the fourth time duration;
• S11) controlling the air pump to pump the external air into the gas channel through the drying tube and the catalytic tube and finally into the CO2 gas chamber through its inlet port, and then expelling the air in the CO2 gas chamber through the exhaust port of the CO2 gas chamber in the first time duration;
• wherein the method for measuring the lifespan of a red blood cell further comprising following step S12 implemented on a first gas channel system after the implementation of the steps S7-S11:
• S12) controlling the air pump to pump the external air into the gas channel of the first gas channel system through the drying tube and the catalytic tube and finally into the CO gas chamber through its inlet port, and then expelling the air in the CO gas chamber through the exhaust port of the same CO gas chamber in a second time duration.

A device for measuring the lifespan of a red blood cell comprising a gas chamber unit, a transmission unit, a circuit unit, a power source unit, a cleaning gas preparation unit, as well as a gas channel system and electrical connections connecting each unit;

• wherein the gas chamber unit comprises a CO gas chamber assembly and a CO2 gas chamber assembly which are both provided with an exhaust port and an inlet port with a solenoid valve;
• the transmission unit comprises a cylinder piston assembly pushed by a lead screw, a stepper motor assembly which drives the lead screw to rotate;
• the cleaning gas preparation unit comprises a gas pump assembly, a drying tube assembly and a catalytic tube assembly;
• and the circuit unit comprises a signal amplification, data processing, and control circuit including a programmable chip;
• the power source unit provides an electric source for satisfying each unit;
• the gas channel system consists of gas channels which comprise connection gas pipes in assemblies in each unit or between the unit assemblies and gas bags, and solenoid valves controlling an on-off state of the gas channel;
• wherein the gas bag comprises a pulmonary alveolus gas bag used for loading pulmonary alveolus gas sampled from subjects, a background gas bag used for loading environment gas sampled at a place where the subjects are located, a first and second reversing gas bags used as an absorption bag for removing ingredients in the pulmonary alveolus gas and the environment gas which interfere CO measurement;
• wherein the pulmonary alveolus gas bag, the background gas bag, the first reversing gas bag and the second reversing gas bag which are connected with the gas chamber unit, the transmission unit and the cleaning gas preparation unit via the gas channels together form one measurement channel.

A method for measuring the lifespan of a red blood cell comprising following steps:

• preparing cleaning gas to clean gas chamber until the gas chamber is full of cleaning gas;
• delivering gas sample to be determined with a small amount each time at an uniform velocity, or for multiple times of intermittence with a small amount each time at an uniform velocity to the gas chamber;
• removing interference components by an interference component absorption bag;
• obtaining endogenous CO concentration of the pulmonary alveolus gas from pulmonary alveolus gas sample and background gas sample by a pair of measurements which are implemented closely in time via a pair of level difference-concentration difference fitting method;
• measuring CO concentration and CO2 concentration by a same instrument simultaneously via a double-gas chamber method using a CO gas chamber and a CO2 gas chamber and correcting an influence of air mixed during a pulmonary alveolus gas sampling process according to the measured CO2 concentration to obtain an accurate value of the endogenous CO concentration of the pulmonary alveolus gas for finally calculating the lifespan of the red blood cell.