Loading patent…
AssigneeViaCyte, Inc.
InventorsKevin Allen D'Amour, Alan D. Agulnick, Susan Eliazer, Emmanuel E. Baetge
Estimating the term…
Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed.
1-20. (canceled)
An in vitro cell culture method for producing human anterior foregut endoderm cells, comprising, contacting a population of human pluripotent stem cells with an effective amount of a retinoid to produce human anterior foregut endoderm cells.
An in vitro cell culture method for producing human midgut endoderm cells, comprising, contacting a population of human pluripotent stem cells with an effective amount of a retinoid to produce human midgut endoderm cells.
An in vitro cell culture method for producing human hindgut endoderm cells, comprising, contacting a population of human pluripotent stem cells with an effective amount of a retinoid to produce human hindgut endoderm cells.
An in vitro method for producing anterior foregut endoderm cells, midgut endoderm cells and/or hindgut endoderm cells, comprising contacting a human pluripotent stem cell population with a retinoid in an amount sufficient to produce the human anterior foregut endoderm cells, midgut endoderm cells and/or human hindgut endoderm cells.
Dependencies are inferred from the claim text.