ISOLATION OF EXTRACELLULAR VESICLES (EVs) FROM RED BLOOD CELLS FOR GENE THERAPY
AssigneeCity University of Hong Kong
InventorsThi Nguyet Minh Le, Jiahai Shi, Muhammad Waqas
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Abstract
A method of RNA delivery using extracellular vesicles (EVs) derived from red blood cells (RBCs). The method comprises the purification and electroporation of the EVs and applying the RNA-loaded EVs to target cells. The method further comprises the treatment of cancer using the RNA-loaded EVs.
Claims24
A method for RNA delivery to target cells comprising:
- a) purification of extracellular vesicles (EVs) from red blood cells (RBCs);
- b) electroporation of the EVs with RNAs to form RNA-loaded EVs;
- and
- c) applying the RNA-loaded EVs to the target cells.
A method for delivery of an antisense oligonucleotide (ASO) to target cells to suppress gene expression, wherein the method comprises:
- a) purification of extracellular vesicles (EVs) from red blood cells (RBCs);
- b) electroporation of the EVs with RNAs to form RNA-loaded EVs;
- and
- c) applying the RNA-loaded EVs to the target cells.
A method of RNA delivery to target cells for a CRISPR genome editing system comprising:
- a) purification of extracellular vesicles (EVs) from red blood cells (RBCs);
- b) electroporation of the EVs with RNAs to form RNA-loaded EVs;
- and
- c) applying the RNA-loaded EVs to the target cells.
A method of treating cancer by delivery of RNA to target cells comprising:
- a) purification of extracellular vesicles (EVs) from red blood cells (RBCs);
- b) electroporation of the EVs with RNAs to form RNA-loaded EVs;
- and
- c) applying the RNA-loaded EVs to the target cells thereby inhibiting the growth of the target cells, wherein the target cells comprise cancer cells.
Dependencies are inferred from the claim text.