Methods and devices for detecting methanol poisoning using formate oxidase
AssigneeOslo Universitetssykehus Hf
InventorsKnut Erik HOVDA, Gaut GADEHOLT, Dag JACOBSEN
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Abstract
The present disclosure relates to compositions and methods for diagnosis, research, and screening for chemicals in biological fluids (e.g., related to methanol poisoning). In particular, the present disclosure relates to point of care systems and methods for detecting formic acid or formate, in biological fluids by means of natural or recombinant formate oxidase.
Claims24
1-16. (canceled)
A method for detecting methanol poisoning in a subject suspected of being poisoned with methanol, comprising:
- a) contacting a biological sample from said subject with a formate oxidase enzyme wherein said formate oxidase acts on formate in said biological sample to produce hydrogen peroxide;
- and
- b) detecting said hydrogen peroxide by producing a colored reagent by reacting said hydrogen peroxide with a peroxidase enzyme and an indicator dye precursor to produce said colored reagent;
- wherein the production of the colored reagent is indicative of methanol poisoning in the subject.
A method for detecting formate in a biological sample from a subject, comprising:
- a) contacting a biological sample with formate oxidase such that formate in said biological sample reacts with said formate oxidase to generate hydrogen peroxide;
- and
- b) detecting said hydrogen peroxide.
A device comprising a substrate having thereon dried formate oxidase in an amount sufficient to oxidize formate in a biological sample when contacted with said biological sample in the presence of a reporting system to produce a detectable signal corresponding the presence of formate in the sample.
37-56. (canceled)
An assay device, comprising:
- a porous substrate comprising a) a formate oxidase polypeptide;
- b) a peroxidase enzyme;
- and c) an indicator dye precursor.
58-62. (canceled)
Dependencies are inferred from the claim text.