Purification of respiratory syncytial virus
AssigneeSanofi Pasteur Biologics, LLC
InventorSophia Tara MUNDLE
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Abstract
Disclosed herein are methods for the purification of Respiratory Syncytial Virus (RSV) particles from a host cell culture comprising treating the host cell culture with an endonuclease, filtering the material to remove cellular debris and/or aggregated material, applying the material to a core bead chromatography resin, and recovering the purified RSV particles. Also disclosed herein are pharmaceutical compositions comprising purified RSV.
Claims21
A method for the purification of Respiratory Syncytial Virus (RSV) particles from a host cell culture comprising RSV particles, the method comprising the steps of:
- a) treating the host cell culture with an endonuclease;
- b) filtering the material from step (a) to remove cellular debris and/or aggregated material;
- c) applying the material obtained from step (b) to a core bead chromatography resin such that the RSV particles flow through the resin;
- and
- d) recovering the purified RSV particles.
A pharmaceutical composition comprising Respiratory Syncytial Virus (RSV) produced in a cell culture, said RSV isolated by the method comprising the steps of:
- a) treating the host cell culture with an endonuclease;
- b) filtering the material from step (a) to remove cellular debris and/or aggregated material;
- c) applying the material obtained from step (b) to a core bead chromatography resin such that the RSV particles flow through the resin;
- d) recovering the purified RSV particles;
- and
- e) suspending the purified RSV particles in a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.