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US 2018/0251755 A1Published application
Methods For Purification of Messenger RNA
AssigneeTranslate Bio, Inc.
InventorsJonathan Abysalh, Daniel Crawford, Frank DeRosa, Shrirang Karve, Anusha Dias, Michael Heartlein
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Abstract
The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.
Claims29
A method of purifying mRNA, comprising steps of:
- providing a solution comprising mRNA;
- adding one or more agents that promote precipitation of mRNA, thereby obtaining a slurry;
- stirring the slurry prior to and/or while providing pressure to the slurry and/or a vacuum to the slurry sufficient to direct the slurry's mother liquor through a filter, thereby obtaining a precipitate-containing composition;
- and
- washing the precipitate-containing composition, thereby yielding a purified mRNA precipitate.
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Dependencies are inferred from the claim text.