Ehv insertion site orf70
AssigneeBoehringer Ingelheim Vetmedica GmbH
InventorsAlice MUNDT, Andreas GALLEI, Kristina REHMET
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of (vector) vaccines, and especially to the novel EHV insertion site ORF70. The present invention further concerns related expression cassettes and vectors, which are suitable to express genes of interest, especially antigen encoding sequences. The viral vectors of the present invention are useful for producing an immunogenic composition or vaccine.
Claims26
An expression cassette comprising
- (i) at least one exogenous nucleotide sequence of interest, preferably a gene of interest, more preferably an antigen encoding sequence, whereby said nucleotide sequence of interest, preferably a gene of interest, more preferably an antigen encoding sequence is operably linked to a promoter sequence, and
- (ii) at least one left ORF70 flanking region selected from the group consisting of:
- SEQ ID NO.:
- 13 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, SEQ ID NO.:
- 15 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, and SEQ ID NO.:
- 17 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, and
- (iii) at least one right ORF70 flanking region selected from the group consisting of:
- SEQ ID NO.:
- 14 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, SEQ ID NO.:
- 16 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, and SEQ ID NO.:
- 18 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof.
An Equid Alphaherpesvirus 1 (EHV-1) vector, preferably strain RacH, comprising
- (i) at least one exogenous nucleotide sequence of interest, preferably a gene of interest, more preferably an antigen encoding sequence, whereby said nucleotide sequence of interest, preferably a gene of interest, more preferably an antigen encoding sequence, is operably linked to a promoter sequence, and
- (ii) at least one left ORF70 flanking region selected from the group consisting of:
- SEQ ID NO.:
- 13 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, SEQ ID NO.:
- 15 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, and SEQ ID NO.:
- 17 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, and
- (iii) at least one right ORF70 flanking region selected from the group consisting of:
- SEQ ID NO.:
- 14 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, SEQ ID NO.:
- 16 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof, and SEQ ID NO.:
- 18 and a 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous and/or identical sequence thereof.
An Equid Alphaherpesvirus 1 (EHV-1) vector, preferably strain RacH, comprising a nucleotide sequence of interest, preferably a gene of interest, more preferably an antigen encoding sequence, inserted into ORF70.
An Equid Alphaherpesvirus 1 (EHV-1) vector, preferably strain RacH, comprising a first nucleotide sequence or gene of interest, preferably an antigen encoding sequence, inserted into ORF70 and a second nucleotide sequence or gene of interest, preferably another antigen encoding sequence, inserted into a second insertion site, preferably ORF1/3.
Dependencies are inferred from the claim text.