Multifunctional magneto-polymeric nanosystems for rapid targeting, isolation, detection and simultaneous imaging of circulating tumor cells
OwnerJayant Jagannath Khandare, Shashwat Banerjee +2inventors
InventorsJayant Jagannath Khandare, Shashwat Banerjee, Muralidhara Padigaru, Ganesh Khutale
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Abstract
A biofunctional multicomponent nanosystem for specific targeting, rapid isolation and simultaneous high resolution imaging of cancer cells is disclosed.
Claims10
1) A biofunctional multicomponent nanosystem comprising:
- (i) cells or antibodies selected from the group consisting of transferrin (Tf)/anti EpCam, CTC specific antibody targeting cancer cells and antibodies associated with cell or proteins for biomolecule interactions;
- (ii) iron oxide (Fe3O4) nanoparticles;
- (iii) cyanine 5 NHS (Cy5) near infrared probe;
- (v) carbon allotropes for interaction with cancer cells;
- (vi) poly(N-isopropylacrylamide) (PNIPAM);
- (vii) fourth generation (G4) dendrimers or polymers selected from the group consisting of polyglycerols, polyamines and hyperbranched polymers;
- and
- (viii) glutathione (GSH) for specific targeting, isolation and imaging of circulating cancer cells
4) A process of synthesizing a biofunctional multicomponent nanosystem comprising the steps of:
- a) synthesizing Fe3O4 magnetic nanoparticles by co-precipitating Fe2+ and Fe3+ ions by ammonia solution and treating under hydrothermal conditions;
- b) anchoring of glutathione with Fe3O4;
- c) synthesis of Fe3O4-GSH-PAMAM G4 dendrimer conjugate;
- d) synthesis of Fe3O4-GSH-PAMAM G4-CNT or graphene conjugate;
- e) synthesis of Fe3O4-GSH-PAMAM G4-CNT or graphene-Cy5-Tf conjugate;
- and
- f) synthesis of Fe3O4-GSH-PAMAM G4-CNT or graphene-Cy5-anti EpCam conjugate.
5) A process of synthesizing a biofunctional multicomponent nanosystem comprising the steps of:
- a) synthesizing Fe3O4 magnetic nanoparticles by co-precipitating Fe2+ and Fe3+ ions by ammonia solution and treating under hydrothermal conditions;
- b) anchoring of glutathione with Fe3O4;
- c) synthesis of Fe3O4-GSH-PAMAM G4 dendrimer conjugate;
- d) synthesis of Fe3O4-GSH-PAMAM G4-CNT or graphene conjugate;
- e) synthesis of Fe3O4-GSH-PAMAM G4-CNT or graphene PNIPAM conjugate
- f) synthesis of Fe3O4-GSH-PAMAM G4-CNT or graphene PNIPAM-Tf conjugate;
- and
- g) synthesis of Fe3O4-GSH-PAMAM G4-CNT or graphene PNIPAM-anti EpCam conjugate.
6) A biofunctional multicomponent nanosystem comprising:
- (i) transferrin (Tf)/anti EpCam or any other CTC specific antibody or biomolecules interacting antibodies specifically targeting cancer cells;
- (ii) iron oxide (Fe3O4) nanoparticles to allow magnetic isolation;
- (iii) cyanine 5 NHS (Cy5) near infra red probe to enable simultaneous high-resolution imaging of the isolated CTCs;
- (v) carbon allotrope for interaction with cancer cells;
- (vi) poly(N-isopropylacrylamide) (PNIPAM), capable of affecting conformational structural changes and solubility enhancer, resulting in cancer cell capture;
- (vii) fourth generation (G4) dendrimers or polymers selected from the group consisting of polyglycerols, polyamines and hyperbranched polymers;
- and
- (viii) glutathione (GSH) for specific targeting, isolation and imaging of circulating cancer cells.
A kit comprising a biofunctional multicomponent nanosystem comprising cells or antibodies selected from the group consisting of transferrin (Tf)/anti EpCam, CTC specific antibody targeting cancer cells and antibodies associated with cell or proteins, iron oxide (Fe3O4) nanoparticles, cyanine 5 NHS (Cy5) near infra red probe, carbon allotropes, poly(N-isopropylacrylamide) (PNIPAM), fourth generation (G4) dendrimers or polymers like polyglycerols/polyimines and glutathione (GSH).
Dependencies are inferred from the claim text.