Compositions and methods of cell attachment

A method of culturing cells, comprising:

• a) providing a microfluidic device comprising a microchannel comprising a surface, said microchannel in fluidic communication with a fluid source comprising fluid;
• b) covalently attaching a bifunctional crosslinker to said surface to create attached crosslinker, c) covalently attaching one or more proteins or peptides to said attached crosslinker as to create a treated surface;
• d) seeding viable cells on said treated surface so as to create attached cells;
• e) flowing fluid from said fluid source through said microchannel so as to create flowing conditions;
• and f) culturing said attached cells under said flow conditions such that said cells remain attached and viable for at least 7 days.

A kit comprising:

• a) a microfluidic device comprising a surface;
• b) a crosslinker comprising at least one light-reactive portion, and at least one chemically reactive portion;
• c) at least one protein or peptide;
• and
• d) a set of instructions.