Electrophoresis gel cassette and comb

An apparatus for gel electrophoresis comprising a comb having wedge-shaped teeth configured to create wedge-shaped sample wells in a gel by insertion of the comb into the gel and by removal of the comb once the gel has been cast, wherein the wedge-shaped teeth have at least one surface extending from a plane parallel to the bottom surface of the teeth at angle of between 45 degrees and 90 degrees to the top of the teeth.

The apparatus of claim 1, wherein the wedge-shaped teeth have flat surfaces at the tips of the teeth.

The apparatus of claim 1, wherein the wedge-shaped teeth are configured to create the wedge-shaped sample wells with larger openings to the wedge-shaped sample wells at the at the top of the wedge-shaped sample wells.

The apparatus of claim 1, wherein the wedge-shaped teeth are configured to create the wedge-shaped sample wells with a volume at least 10% or at least 100% greater than a volume of sample wells created by using a standard comb.

The apparatus of claim 4, wherein the volume of sample wells created by using the standard comb is about 37.5 μL.

The apparatus of claim 1, wherein the wedge-shaped teeth are configured to create the wedge-shaped sample wells with a volume of at least 40 μL, at least 41 μL or at least 70 μL.

The apparatus of claim 1, wherein the wedge-shaped teeth are configured to create the wedge-shaped sample wells with a greater cross sectional area at the top of the wedge-shaped sample wells as compared to the bottom of the wedge-shaped sample wells.

The apparatus of claim 1, wherein the comb is a ten-tooth comb for forming ten wedge-shaped sample wells, forming twelve wedge-shaped sample wells, forming fifteen wedge-shaped sample wells, forming seventeen wedge-shaped sample wells, or forming twenty wedge-shaped sample wells.

The apparatus of claim 1, wherein the comb is molded from a plastic comprising:

• polyethylene terephthalate, polyvinyl chloride, polymethyl methacrylate, polystyrene, polyethylene, polymethyl polypropylene, cellulose acetates, copolymers, or polycarbonate.

The apparatus of claim 1, wherein the wedge-shaped teeth have a top that is between about 0.15 inch and about 0.2 inch in depth.

The apparatus of claim 1, further comprising a gel cassette with an opening into which the comb is insertable.

The apparatus of claim 1, wherein the apparatus is configured to create a gel having sharp bands or a gel having high resolution.

The apparatus of claim 1, wherein the wedge-shaped teeth have at least one surface extending from a plane parallel to the bottom surface of the teeth at angle of 70 degrees.

The apparatus of claim 1, comprising a recessed area on the comb.

The apparatus of claim Error! Reference source not found, wherein the cassette has at least one interior surface.

An apparatus for gel electrophoresis comprising a comb having wedge-shaped teeth configured to create wedge-shaped sample wells in a gel by insertion of the comb into the gel and by removal of the comb once the gel has been cast, wherein the wedge-shaped teeth have at least one surface extending from a plane at the bottom surface of the teeth at an angle of between 45 degrees and 90 degrees to the top of the teeth.