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US 2018/0024030 A1Published application
Detection of low concentration biological agents
AssigneeThe Regents of the University of California
InventorsMichelle KHINE, Himanshu SHARMA, Sophia LIN, Jolie McLane
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Abstract
Provided are methods of preparing a sample for detection by placing the sample on a shrinkable scaffold and then shrinking the scaffold. An exemplary shrinkable scaffold is a thermoplastic substrate.
Claims14
A method for preparing a sample for detection of a fluorescently labeled polynucleotide suspected to be present in the sample by detecting the amount of fluorescence emitted by the fluorescently labeled polynucleotide, comprising:
- covalently attaching the polynucleotide on a silica surface of a thermoplastic material, the silica surface having been deposited by e-beam vaporization or sputtering,
- heating and shrinking the thermoplastic material thereby concentrating the sample on the silica surface and to provide a rough porous substrate that results in an optical effect of light scattering.
Dependencies are inferred from the claim text.