Methods and nucleic acids for analyses of cell proliferative disorders

A method for detecting CpG methylation of FOXL2 in genomic DNA from a biological sample isolated from a subject having lung cancer, the method comprising:

• (a) treating genomic DNA isolated from a biological sample obtained from the subject with at least one reagent or series of reagents that distinguishes between methylated and non-methylated CpG dinucleotides within at least one target region of the genomic DNA,
• wherein the biological sample is selected from the group consisting of cells or cell lines, histological slides, biopsies, paraffin-embedded tissue, body fluids, blood plasma, blood serum, whole blood, isolated blood cells, sputum and biological material derived from the oral epithelium or from the lung, comprising bronchial lavage, bronchial alveolar lavage, bronchial brushing, and bronchial abrasion, and combinations thereof,
• wherein the at least one target region of the genomic DNA comprises or is complementary to a sequence of at least 16 contiguous nucleotides of SEQ ID NO:
• 5, and wherein said contiguous nucleotides comprise at least one CpG dinucleotide sequence;
• and
• (b) detecting the presence or absence of CpG methylation within the target region.