Loading patent…
AssigneePacific Biosciences of California, Inc.
InventorsJeremiah Hanes, Satwik Kamtekar, Yufeng Qian, Keith Bjornson, Thang Pham, David Rank, Paul Peluso, Erik Miller, Kristin Gleitsman, Leewin Chern, Steven Lin, Stephen Turner
Estimating the term…
The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in an active loading of molecules and complexes into reaction sites with improved efficiency over loading by passive diffusion methods alone.
1-77. (canceled)
A method of delivering nucleic acids to reaction sites, the method comprising:
The method of claim 106, wherein the releasing step comprises applying trypsin to the coated surface.
The method of claim 106, wherein the positively charged peptide or polypeptide is a member selected from the group consisting of Tat, poly-lysine, poly-arginine, and histone.
The method of claim 106, wherein the positively charged peptide or polypeptide comprises a Tat peptide.
92-105. (canceled)
Dependencies are inferred from the claim text.