Biomolecule sequencing devices, systems and methods

A system for detecting a biomolecule using at least one pair of nanogap electrodes, comprising:

• a fluid flow unit for directing a plurality of monomers with reference substances bound thereto to the at least one pair of nanogap electrodes;
• and
• a computer processor coupled to the at least one pair of nanogap electrodes and programmed to:
• (a) measure tunneling nanocurrent signals resulting from the plurality of monomers with reference substances bound thereto using the at least one pair of nanogap electrodes, wherein the tunneling nanocurrent signals correspond to the reference substances;
• and
• (b) identify a monomer of the plurality of monomers with reference substances bound thereto based on the tunneling nanocurrent signals measured in (a).

A method of detecting a biomolecule, comprising:

• (a) providing a plurality of monomers with reference substances bound thereto;
• (b) using at least one pair of nanogap electrodes to measure tunneling nanocurrent signals resulting from the plurality of monomers with reference substances bound thereto, the tunneling nanocurrent signals corresponding to the reference substances;
• and
• (c) identifying a monomer of the plurality of monomers with reference substances bound thereto based on the tunneling nanocurrent signals measured in (b).