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US 2018/0023127 A1Published application
Visually determinable genetic testing
AssigneeKurashiki Boseki Kabushiki Kaisha
InventorsHiroki YAMASHITA, Kazuhiko KOGOH, Shuichi OMURA
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Abstract
The purpose of the present invention is to provide a DNA detection technique by which testing can be simply and accurately performed at clinical sites or the like. The present invention provides a gene analysis method which employs, as a detection sample, a single strand nucleotide product that is amplified by ligation, and which can be performed visually in a more simple and rapid way.
Claims7
A genetic analysis method comprising hybridizing the following (i) and (ii) and making the hybridized product visually detectable:
- (i) an oligonucleotide probe immobilized on a strip;
- (ii) a single-stranded ligation product amplified by subjecting the following (a) and (b) to ligation treatment through one or a plurality of theiinal cycling treatments using a nucleic acid sample as a template:
- (a) a query oligo comprising a first single-stranded oligonucleotide complementary to said nucleic acid sample and a tag sequence capable of hybridizing with said probe or a labeling moiety;
- (b) a common oligo comprising a second single-stranded oligonucleotide complementary to said nucleic acid sample and adjacent to said first single-stranded oligonucleotide and a tag sequence or a labeling moiety.
1 more element
Dependencies are inferred from the claim text.