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US 2018/0023120 A1Published application
Methods for Preparative In Vitro Cloning
AssigneeGinkgo Bioworks, Inc.
InventorsLi-yun A. Kung, Daniel Schindler, Joseph Jacobson
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Abstract
Methods and devices relate to the isolation of nucleic acids of interest from within a population of nucleic acids such as libraries of nucleic acid sequences.
Claims20
A method of isolating a target nucleic acid, the method comprising:
- (a) providing a population of nucleic acid molecules comprising a plurality of distinct nucleic acid molecules;
- (b) providing a plurality of oligonucleotide tags;
- (c) attaching the oligonucleotide tags to one terminus of the plurality nucleic acid molecules thereby generating a subpopulation of nucleic acid-oligonucleotide tag molecules;
- (d) amplifying the plurality of nucleic acid-oligonucleotide tag molecules using primers complementary to the plurality of oligonucleotide tags;
- and
- (e) isolating at least one target nucleic acid-oligonucleotide tag molecule of the subpopulation of nucleic acid-oligonucleotide tag molecules.
3 more elements
A method of isolating a target nucleic acid, the method comprising:
- (a) providing a population comprising a plurality of different nucleic acid molecules;
- (b) providing a plurality of microparticles, wherein each microparticle has an oligonucleotide sequence complementary to a portion of the nucleic acid molecules immobilized on its surface;
- (c) forming a population of nucleic acid molecules hybridized to the complementary oligonucleotide sequence on the microparticles;
- and
- (d) isolating at least one target nucleic acid molecule.
2 more elements
A method of isolating a target nucleic acid, the method comprising:
- (a) providing a population comprising a plurality of different nucleic acid molecules;
- (b) providing a dilution of the population of nucleic acid molecules;
- (c) separating the nucleic acid molecules into samples comprising a single nucleic acid molecule or a smaller number of nucleic acid molecules;
- (d) amplifying the nucleic acid molecules from step c thereby forming amplified nucleic acid molecules;
- (e) optionally repeating step c and d in the samples that do not comprise a nucleic acid molecule;
- and
- (f) isolating at least one target nucleic acid molecule.
4 more elements
Dependencies are inferred from the claim text.