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AssigneeInvista North America S.A.R.L.
InventorsStephen Cartman, Jonathan Combe, Alexander B. Foster, Jonathan Kennedy
Estimating the term…
This disclosure relates to genome-scale attenuation or knockout strategies for directing carbon flux to certain carbon based building blocks within the 7-aminoheptanoic acid (7-AHA) and 6-aminohexanoic acid (6-AHA) biosynthesis pathways, for example, to achieve reduced flux to unwanted side products while achieving increased production of desired intermediates and end products. This disclosure also relates to non-naturally occurring mutant bacterial strains comprising one or more gene disruptions in aldehyde reductase and/or aldehyde dehydrogenase genes that are generated to direct carbon flux to certain carbon based building blocks. This disclosure further relates to a method for enhancing production of carbon based building blocks by generating non-naturally occurring mutant bacterial strains, culturing said mutant bacterial strains in the presence of suitable substrates or under desired growth conditions, and substantially purifying the desired end product.
A method for enhancing biosynthesis of 7-aminoheptanoic acid or 6-aminohexanoic acid comprising:
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A recombinant host comprising one or more gene attenuations in aldehyde reductase and/or aldehyde dehydrogenase genes, wherein said gene attenuations reduce aldehyde reductase and/or aldehyde dehydrogenase activity of polypeptides encoded by said genes, and wherein said recombinant host produces an increased level of 7-aminoheptanoic acid converted from pimelic acid or pimelic acid derivatives or 6-aminohexanoic acid converted from adipic acid or adipic acid derivatives as compared to a wild-type recombinant host.
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A non-naturally occurring organism comprising at least one exogenous nucleic acid encoding at least one polypeptide having the activity of at least one enzyme depicted in any one of FIGS. 1 to 5 or FIG. 12.
A nucleic acid construct or expression vector comprising a polynucleotide encoding:
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A non-naturally occurring biochemical network comprising a 5-hydroxypentanoyl-CoA, an exogenous nucleic acid encoding a polypeptide having the activity of a β-ketothiolase classified under EC. 2.3.1, and a 3-oxo-7-hydroxyheptanoyl-CoA.
Dependencies are inferred from the claim text.