Materials and methods utilizing biotin producing mutant hosts for the production of 7-carbon chemicals

A recombinant host for producing pimeloyl-ACP, wherein:

• said recombinant host comprising a deletion of a bioF gene;
• said recombinant host expresses a BioF at a level below that of a wild type host;
• said recombinant host does not express BioF activity;
• or
• said recombinant host comprises at least one exogenous nucleic acid encoding bioW and a CoA-specific bioF.

A nucleic acid construct or expression vector comprising:

• a polynucleotide encoding a polypeptide having 8-amino-7-oxononanoate synthase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having 8-amino-7-oxononanoate synthase activity is a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 23;
• a polypeptide having thioesterase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having thioesterase activity is chosen from:
• (a) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 1;
• (b) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 21;
• and (c) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 22;
• a polynucleotide encoding a polypeptide having carboxylate reductase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having carboxylate reductase activity is chosen from:
• (a) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 2:
• (b) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 3;
• (c) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 4;
• (d) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 5;
• (e) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 6;
• and (f) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 7;
• a polynucleotide encoding a polypeptide having ω-transaminase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having ω-transaminase activity is chosen from:
• (a) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 8:
• (b) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 9;
• (c) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 10 (d) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 11:
• (e) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 12;
• and (f) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 13;
• a polynucleotide encoding a polypeptide having phosphopantetheinyl transferase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having phosphopantetheinyl transferase activity is chosen from:
• (a) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 14 and (b) a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 15;
• a polynucleotide encoding a polypeptide having pimeloyl-ACP methyl ester esterase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having pimeloyl-ACP methyl ester esterase activity is a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 17;
• a polynucleotide encoding a polypeptide having 6-carboxyhexanoate-CoA ligase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having 6-carboxyhexanoate-CoA ligase activity is a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 18;
• a polypeptide having 8-amino-7-oxononanoate synthase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having 8-amino-7-oxononanoate synthase activity is a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 19;
• or
• a polynucleotide encoding a polypeptide having malonyl-ACP O-methyltransferase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having malonyl-ACP O-methyltransferase activity is a polypeptide having at least 70% sequence identity or homology to the polypeptide of SEQ ID NO:
• 20.