Processes for producing fermentation products
AssigneeNovozymes A/S
InventorsRandy Deinhammer, Suzanne Clark, Mauricio Quiros, John Matthews, Anne Glud Hjulmand, Chee Leong Soong, Tomoko Matsui, Shinobu Takagi
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Abstract
The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.
Claims20
A process for producing fermentation products from starch-containing material comprising the steps of:
- i) liquefying the starch-containing material at a pH in the range from 4.5-5.0 at a temperature in the range from 80-90° C. using:
- a thermostable bacterial alpha-amylase and
- a thermostable bacterial protease having a thermostability value of more than 20% determined as Relative Activity at 80° C./70° C.;
- ii) saccharifying using a glucoamylase;
- and
- iii) fermenting using a fermenting organism.
The process of claim 4, wherein the alpha-amylase variant has at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 91%, more preferably at least 92%, even more preferably at least 93%, most preferably at least 94%, and even most preferably at least 95%, such as even at least 96%, at least 97%, at least 98%, at least 99%, but less than 100% identity to the mature part of the polypeptide of SEQ ID NO:
- 1 herein.
A process for producing fermentation products from starch-containing material comprising the steps of:
- i) liquefying the starch-containing material at a pH in the range from 4.5-5.0 at a temperature in the range from 80-90° C. using:
- a thermostable bacterial alpha-amylase having a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl2) of at least 10, wherein the alpha-amylase is truncated and has altered amino acids selected from the group consisting of:
- I181*+G182*+N193F+V59A+Q89R+E129V+K177L+R179E+H208Y+K220P+N224L+Q254S;
- I181*+G182*+N193F+E129V+K177L+R179E;
- and
- I181*+G182*+N193F+E129V+K177L+R179E+K220P+N224L+S242Q+Q254S, using SEQ ID NO:
- 1 for numbering;
- a Pyrococcus protease having at least 90% identity to SEQ ID NO:
- 13 and a thermostability value of more than 20% determined as Relative Activity at 80° C./70° C.;
- ii) saccharifying using a carbohydrate-source generating enzyme;
- iii) fermenting using a fermenting organism.
Dependencies are inferred from the claim text.