Recombinant aav variants and uses thereof
AssigneeUniversity of Massachusetts
InventorsGuangping Gao, Jun Xie, Terence Flotte
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Abstract
The disclosure in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the disclosure relates to gene transfer methods using the recombinant adeno-associated viruses. In some aspects, the disclosure relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.
Claims42
A recombinant expression vector comprising a sequence selected from the group consisting of:
- SEQ ID NO:
- 1 to 47, or a fragment thereof that does not encode a peptide that is identical to a sequence of any one of SEQ ID NOs:
- 98 to 100.
An isolated AAV capsid protein comprising an amino acid sequence selected from the group consisting of:
- SEQ ID NOs:
- 51 to 97.
An isolated AAV capsid protein comprising a sequence selected from the group consisting of:
- SEQ ID NOs:
- 51 to 61, wherein an amino acid of the sequence that is not identical to a corresponding amino acid of the sequence set forth as SEQ ID NO:
- 98 is replaced with a conservative substitution.
An isolated AAV capsid protein comprising a sequence selected from the group consisting of:
- SEQ ID NOs:
- 62 to 67, wherein an amino acid of the sequence that is not identical to a corresponding amino acid of the sequence set forth as SEQ ID NO:
- 99 is replaced with a conservative substitution.
An isolated AAV capsid protein comprising a sequence selected from the group consisting of:
- SEQ ID NOs:
- 68 to 97, wherein an amino acid of the sequence that is not identical to a corresponding amino acid of the sequence set forth as SEQ ID NO:
- 100 is replaced with a conservative substitution.
A host cell containing a nucleic acid that comprises a coding sequence selected from the group consisting of:
- SEQ ID NO:
- 1 to 47 that is operably linked to a promoter.
A kit for producing a rAAV, the kit comprising:
- a container housing an isolated nucleic acid having a sequence of any one of SEQ ID NO:
- 1 to 47.
An isolated AAV capsid protein encoded by a nucleic acid selected from the group consisting of SEQ ID NO:
- 1 to 47.
Dependencies are inferred from the claim text.