Double strand rna delivery system for plant-sap-feeding insects
OwnerSaikat Kumar B. Ghosh, Dawn E. Gundersen-Rindal +1inventors
InventorsSAIKAT KUMAR B. GHOSH, DAWN E. GUNDERSEN-RINDAL, WAYNE B. HUNTER
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Abstract
The present disclosure provides compositions and methods of delivering double strand ribonucleic acid (dsRNA) to insects that penetrate plant tissues to feed on sap and other liquid components of plants. Taking advantage of the liquid transport capabilities of plant vascular structures, dsRNA is provided to plant tissues in an aqueous solution that is then transported throughout the tissues. The dsRNA-laden plant material is then presented to insects that can ingest the dsRNA by feeding on the plant tissue.
Claims33
A composition comprising a living plant material and at least one double-strand RNA (dsRNA) not produced by the living plant material, wherein the at least one dsRNA is distributed throughout at least part of the living plant material's vascular tissues and wherein the living plant material does not contain genetic information allowing for the production of the at least one double strand dsRNA.
A method of inducing RNA interference (RNAi) in an insect, comprising the steps of:
- a) providing a living plant material containing at least one double-strand RNA (dsRNA) not produced by the living plant material, wherein the at least one dsRNA is distributed throughout at least part of the living plant material's vascular tissues and wherein the living plant material does not contain genetic information allowing for the production of the at least one dsRNA;
- b) allowing the insect to ingest a sufficient amount of the at least one dsRNA by feeding on the plant material to interfere with the production of at least one protein targeted by the at least one dsRNA, thereby inducing RNAi in the insect.
A method of controlling an insect comprising the steps of:
- a) providing a living plant material containing at least one double-strand RNA (dsRNA) not produced by the living plant material, wherein the at least one dsRNA is distributed throughout at least part of the living plant material's vascular tissues and wherein the living plant material does not contain genetic information allowing for the production of the at least one dsRNA;
- b) allowing the insect to ingest a sufficient amount of the at least one dsRNA, by feeding on the plant material, to interfere with the production of at least one protein targeted by the at least one dsRNA, thereby inducing RNAi in the insect, and;
- c) controlling the insect via RNAi.
Dependencies are inferred from the claim text.