G24 glucoamylase compositions and methods
AssigneeFornia BioSolutions, Inc.
InventorsJie Yang, Goutami Banerjee, Xiyun Zhang, Khin Oo, Yingxin Zhang
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Abstract
The invention is directed to variant glucoamylases as compared to SEQ ID NO:1, including amino acid substitutions at positions including 24, 32, 47, 71, 75, 88, 102, 104, 114, 118, 190, 201, 204, 260, 228, 230, 269, 271, 272, 281, 283, 284, 285, 293, 350, 313, 321, 338, 343, 367, 371, 386, 372, 401, 408, 420, 448, 463, 483, 484, 507, 530, 563 599 and 605 that retain glucoamylase activity.
Claims30
A composition comprising a variant glucoamylase enzyme comprising an amino acid substitution as compared to SEQ ID NO:1, wherein said amino acid substitution is at a position selected from the group of:
- 114, 530, 190, 599, 24, 32, 47, 71, 75, 88, 102, 104, 118, 201, 204, 260, 228, 230, 269, 271, 272, 281, 283, 284, 285, 293, 350, 313, 321, 338, 343, 367, 371, 386, 372, 401, 408, 420, 448, 463, 483, 484, 507, 563 and 605, wherein said variant enzyme is at least 95% identical to SEQ ID NO:1, and wherein the variant enzyme retains glucoamylase activity.
A composition comprising a variant glucoamylase enzyme comprising an amino acid substitution as compared to SEQ ID NO:1, wherein said amino acid substitution is at a position selected from the group of:
- 114, 530, 190, 599, 24, 32, 47, 71, 75, 88, 102, 104, 118, 201, 204, 260, 228, 230, 269, 271, 272, 281, 283, 284, 285, 293, 350, 313, 321, 338, 343, 367, 371, 386, 372, 401, 408, 420, 448, 463, 483, 484, 507, 563 and 605, wherein said variant glucoamylase enzyme has at least 1.1 fold increased activity as compared to SEQ ID NO:1 under a condition selected from the group consisting of thermostability at 40° C., thermostability at 52° C., thermostability at 56° C. and thermostability at 60° C., and wherein said variant enzyme is at least 95% identical to SEQ ID NO:1.
(canceled)
Dependencies are inferred from the claim text.