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AssigneeAlliance for Sustainable Energy, LLC
InventorsEllen Lee NEIDLE, Christopher W. JOHNSON, Gregg Tyler BECKHAM, Jeffrey G. LINGER, Mark Andrew EITEMAN, Melissa TUMEN-VELASQUEZ, Alaa Ashraf Mahmoud AHMED
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Disclosed herein are enzymes useful for the dealkylation of aromatic substrates, including the conversion of guaiacol or guaethol to catechol. Methods of converting aromatic substrates found in lignin-based feedstocks such as pyrolysis oil into products such as catechol are also disclosed. Also presented herein are methods for rapidly evolving and optimizing genetic regions.
An isolated DNA molecule encoding a chimeric enzyme comprising a cytochrome P450 polypeptide and a catechol 1,2-dioxygenase polypeptide.
A method for removing an alkyl group from an aromatic substrate, comprising contacting a material containing the aromatic substrate with a chimeric enzyme comprising a cytochrome P450 polypeptide and a catechol 1,2-dioxygenase polypeptide to generate a dealkylation product.
Dependencies are inferred from the claim text.