Reprogramming Method for Producing Induced Pluripotent Stem Cells (iPSC)
AssigneeCentre National de la Recherche Scientifique (CNRS)
InventorPierre Roux
Life of this patent
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Abstract
The present invention is in the field of stem cells. Particularly, the present invention relates to a reprogramming method for producing induced pluripotent stem cells (iPSC) by transducing somatic differentiated cells with a viral vector expressing Δ133p53β isoform, Δ133p53γ isoform or both Δ133p53β and Δ133p53γ isoforms; to induced pluripotent stem cells as produced by this method; to uses thereof in cell therapy and as an in vitro model for studying various diseases; and to a method for detecting iPSC.
Claims14
A reprogramming method for producing induced pluripotent stem cells (iPSC), comprising:
- a) transducing somatic differentiated cells with a vector expressing Δ133p53β isoform, Δ133p53γ isoform or both Δ133p53β and Δ133p53γ isoforms;
- b) culturing transduced somatic differentiated cells in a medium supporting their expansion;
- and
- c) isolating induced pluripotent stem cells.
An in vitro method for detecting Induced pluripotent stem cells (iPSC) comprising detecting the presence of Δ133p53β isoform, Δ133p53γ isoform or both Δ133p53β and Δ133p53γ isoforms by detecting Δ133p53β isoform, Δ133p53γ isoform or both Δ133p53β and Δ133p53γ isoforms polypeptide and/or by detecting Δ133p53β isoform, Δ133p53γ isoform or both Δ133p53β and Δ133p53γ isoforms mRNA and/or a fragment thereof.
Dependencies are inferred from the claim text.