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US 2018/0023055 A1Published application
Method for culturing primary cells
AssigneeOsaka Prefectural Hospital Organization
InventorsNorikatsu MIYOSHI, Shiki FUJINO, Masayuki OHUE, Masayoshi YASUI
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Abstract
Method for producing a primary culture of cancer cells, comprising: (a) fragmenting cancer tissues derived from a living body and removing impurities from the fragmented cancer tissues, (b) subjecting the tissue masses obtained in (a) to suspension culture, and (c) subjecting the culture obtained in (b) to adherent culture.
Claims6
A method for producing a primary culture of cancer cells, comprising:
- (a) fragmenting cancer tissues derived from a living body and removing impurities from the fragmented cancer tissues;
- (b) subjecting the tissue masses obtained in (a) to suspension culture;
- and
- (c) subjecting the culture obtained in (b) to adherent culture.
1 more element
Dependencies are inferred from the claim text.