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US 2018/0023049 A1Published application
Synthetic peptide hydrogel formulations for use as extracellular matrix
Assignee3-D Matrix, Ltd.
InventorsEun Seok Gil, Karl Patrick Gilbert
Life of this patent
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Abstract
Synthetic peptide hydrogel solutions having a pH level of about 3.5 or less and a tonicity within an isotonic osmolality range for use in cell culture experimentation. Related kits and cell culture methods are also disclosed.
Claims33
A cell culture kit, comprising:
- a synthetic peptide hydrogel solution;
- a dilution solution;
- and
- instructions to:
- adjust a concentration of the synthetic peptide hydrogel solution with the dilution solution while maintaining its tonicity at a plasma osmolality level and its pH at a level of about 3.5 or less, and
- conduct a cell culture experiment with the diluted synthetic peptide hydrogel solution.
3 more elements
A cell culture method, comprising:
- maintaining a pH level of a synthetic peptide hydrogel solution at about 3.5 or less;
- maintaining a tonicity of the synthetic peptide hydrogel solution at a plasma osmolality level;
- diluting the peptide hydrogel solution to a predetermined concentration;
- culturing cells in the peptide hydrogel solution for a predetermined period of time;
- and
- isolating the cultured cells.
3 more elements
A synthetic peptide hydrogel solution having a pH level of about 1.5 to about 3.5, and a tonicity at plasma osmolality in a range of about 260 to about 360 mOsm/L with respect to a target species and a target cell type.
Dependencies are inferred from the claim text.