Solventless winterization of microbial oil

A method for winterizing oil comprising the steps of:

• (a) providing an oil;
• (b) heating the oil to a first temperature and maintaining the oil at the first temperature for a first period of time;
• (c) reducing the first temperature of the oil after the first period of time to a second temperature over a second period of time, wherein reducing the first temperature produces a first solid fraction and first liquid fraction of the oil;
• (d) removing the first solid fraction from the oil;
• (e) reducing the second temperature of the first liquid fraction of the oil over a third period of time to a third temperature, wherein reducing the second temperature of the oil produces a second solid fraction and second liquid fraction of the oil, and wherein the oil is not heated between the steps of reducing the first temperature and reducing the second temperature;
• (f) removing the second solid fraction from the oil;
• and
• (g) recovering the second liquid fraction of the oil.