Microspheres of Hydrolysed Starch with Endogenous, Charged Ligands

A method for carrying out hemostasis comprising the step of administering an effective amount of a biodegradable microsphere having a diameter of 10 to 2000 μm comprising cross-linked hydrolysed starch onto which at least one type of ligand, selected from the group consisting of amino acids and nitrogen containing organic acids, has been coupled via a carboxylic ester bond formed between the carboxylic acid group of the ligand and a hydroxyl group of a glucose residue in the cross-linked hydrolysed starch, wherein said ligand is an endogenous, charged molecule with a molecular mass of less than 1000 Da comprising at least one additional amine function, said ligand being positively charged or zwitter ionic, and wherein on average 0.05 to 1.5 ligands have been coupled to each glucose moiety in the hydrolysed starch, to a mammal suffering from a bleeding wound, thereby carrying out hemostasis.

A method for carrying out wound healing comprising administering an effective amount of a material comprising biodegradable microspheres having a diameter of 10 to 2000 μm comprising cross-linked hydrolysed starch onto which at least one type of ligand, selected from the group consisting of amino acids and nitrogen containing organic acids, has been coupled via a carboxylic ester bond formed between the carboxylic acid group of the ligand and a hydroxyl group of a glucose residue in the cross-linked hydrolysed starch, wherein said ligand is an endogenous, charged molecule with a molecular mass of less than 1000 Da comprising at least one additional amine function, said ligand being positively charged, wherein on average 0.05 to 1.5 ligands have been coupled to each glucose moiety in the hydrolysed starch, and wherein said microspheres forms a three-dimensional structure comprising voids between the microspheres, to a mammal suffering from a wound, thereby carrying out wound healing.