Nanoparticle-protein complex for intracellular protein delivery

A nanoparticle-protein complex comprising:

• a gold nanoparticle comprising an amine-containing ligand having the structure:
• or a salt thereof, wherein m is 0 to 1,000 and n is 0 to 1,000;
• and
• a protein comprising a carboxylic acid-containing tag having the structure:
• or a salt thereof,
• or,
• or a salt thereof,
• wherein n is about 1 to about 10,000, wherein the protein is chosen from GFP, Granzyme A, mCherry, Cre recombinase, Cas9, Histone 2A, and combinations thereof;
• wherein the nanoparticle-protein complex has a largest dimension of about 50 to about 999 nm, the gold nanoparticle has a largest dimension of about 1 nm to about 50 nm, in solution the protein has a largest dimension of about 0.5 nm to about 50 nm, the gold nanoparticle has about 1 to about 10,000 of the amine-containing ligands, the nanoparticle-protein complex has about 1 to about 10,000 of the gold nanoparticles, and the nanoparticle-protein complex has about 1 to about 10,000 molecules of the protein.

A nanoparticle-protein complex comprising:

• a gold nanoparticle comprising an amine-containing ligand having the structure:
• or a salt thereof, wherein m is 0 to 1,000 and n is 0 to 1,000;
• a protein comprising a carboxylic acid-containing tag having the structure:
• or a salt thereof,
• or,
• or a salt thereof,
• wherein n is about 1 to about 10,000, wherein the protein is chosen from GFP, Granzyme A, mCherry, Cre recombinase, Cas9, Histone 2A, and combinations thereof;
• and
• a nucleic acid material that is gRNA;
• wherein the nanoparticle-protein complex has a largest dimension of about 50 to about 999 nm, the gold nanoparticle has a largest dimension of about 1 nm to about 50 nm, in solution the protein has a largest dimension of about 0.5 nm to about 50 nm, the gold nanoparticle has about 1 to about 10,000 of the amine-containing ligands, the nanoparticle-protein complex has about 1 to about 10,000 of the gold nanoparticles, the nanoparticle-protein complex has about 1 to about 10,000 molecules of the protein, and the nanoparticle-protein complex has about 1 to about 10,000 molecules of the nucleic acid material.