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AssigneesNational University Corporation Kyoto Institute of Technology, Nissan Chemical Industries, Ltd.
InventorsYoichi KUMADA, Ryoko OTSUKI, Ryota AKAI, Junko KATAYAMA, Kazutaka MATOBA
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An object of the present invention is to provide a polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane (PDMS) is bound; a method for immobilizing a target protein on a polydimethylsiloxane substrate; a peptide having an affinity for PDMS; a polynucleotide encoding a peptide having an affinity for PDMS; and a vector using thereof. A polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane is bound, the peptide comprising the following peptide (1a) or (1b), or a fragment thereof: (1a) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9; and (1b) a peptide comprising the amino acid sequence of (1a) in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane.
A polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane is bound,
A method for immobilizing a target protein on a polydimethylsiloxane substrate, the method comprising the step of bringing a peptide having an affinity for polydimethylsiloxane into contact with the polydimethylsiloxane substrate, wherein the peptide is incorporated in the target protein, and the peptide comprises the following peptide (1a) or (1b), or a fragment thereof:
A composition for immobilizing a target protein on a polydimethylsiloxane substrate, the composition comprising a peptide having an affinity for polydimethylsiloxane, the peptide comprising the following peptide (1a) or (1b), or a fragment thereof:
(canceled)
A peptide having an affinity for polydimethylsiloxane, the peptide comprising the following peptide (1c) or (1d), or a fragment thereof:
Dependencies are inferred from the claim text.