Antibodies to risperidone haptens and use thereof
AssigneeJanssen Pharmaceutica NV
InventorsEric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Maarten Vliegen, Pieter Rik Haspeslagh
Life of this patent
Estimating the term…
Abstract
Disclosed is an antibody which binds to risperidone, which can be used to detect risperidone in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of risperidone, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
Claims25
A method of detecting risperidone in a sample, the method comprising:
- (i) generating an antibody or a binding fragment thereof that specifically binds to risperidone in response to a conjugate of a compound of Formula I and an immunogenic carrier,
- Formula I:
- wherein:
- R1 is H, or OH;
- R2 is
- or O(CH2)rNHC(O)(CH2)mCO2H;
- wherein:
- r is 1, 2, 3, 4, or 5;
- m is 1, 2, 3, 4, or 5;
- and
- n is 1, 2, 3, 4, or 5;
- (ii) contacting a sample with the antibody or binding fragment thereof labeled with a detectable marker, wherein the labeled antibody or binding fragment and risperidone present in the sample form a labeled complex;
- and
- (iii) detecting the labeled complex thereby detecting risperidone in the sample.
A competitive immunoassay method for detecting risperidone in a sample, the method comprising:
- (i) generating an antibody or a binding fragment thereof that specifically binds to risperidone in response to a conjugate of a compound of Formula I and an immunogenic carrier,
- Formula I:
- wherein:
- R1 is H, or OH;
- R2 is
- or O(CH2)rNHC(O)(CH2)mCO2H;
- wherein:
- r is 1, 2, 3, 4, or 5;
- m is 1, 2, 3, 4, or 5;
- and
- n is 1, 2, 3, 4, or 5;
- (ii) contacting a sample with the antibody or binding fragment thereof, and with risperidone or a competitive binding partner of risperidone, wherein one of the antibody or binding fragment thereof and the risperidone or competitive binding partner thereof is labeled with a detectable marker, and wherein sample risperidone competes with the risperidone or competitive binding partner thereof for binding to the antibody or binding fragment thereof;
- and
- (ii) detecting the label thereby detecting sample risperidone.
Dependencies are inferred from the claim text.